Free ribonucleoside triphosphates in mouse liver after α-amanitin injection
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منابع مشابه
Ribonucleoside Triphosphates as Substrate of Human Immunodeficiency Virus Type 1 Reverse Transcriptase in Human Macrophages*
We biochemically simulated HIV-1 DNA polymerization in physiological nucleotide pools found in two HIV-1 target cell types: terminally differentiated/non-dividing macrophages and activated/dividing CD4(+) T cells. Quantitative tandem mass spectrometry shows that macrophages harbor 22-320-fold lower dNTP concentrations and a greater disparity between ribonucleoside triphosphate (rNTP) and dNTP c...
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The synthesis of 5'-triphosphates of 4-thiouridine, 2-thiocytidine, 5-bromocytidine, and [6N]adenosine is described. All compounds are characterized by chromatographic and spectral data. Phosphorylation of corresponding nucleotides was performed using phosphoryl chloride and con densation with pyrophosphate. Intermediates were not isolated. The final products were purified by chromatography an...
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The primase fragment of bacteriophage T7 gene 4 protein catalyzes the synthesis of oligoribonucleotides in the presence of ATP, CTP, Mg(2+) (or Mn(2+)), and DNA containing a primase recognition site. During chain initiation, ATP binds with a K(m) of 0.32 mM, and CTP binds with a K(m) of 0.85 mM. Synthesis of the dinucleotides proceeds at a rate of 3.8/s. The dinucleotide either dissociates or i...
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The control of cellular proliferation in mam malian tissues is currently being investigated in terms of the basic biochemical processes leading to deoxyribonucleic acid (DNA) synthesis and the means by which critical steps in the biosynthetic sequence may be regulated by product interaction. Various enzymatic reactions involved in the syn thesis of DNA precursor substances have been ex amined i...
متن کاملElectron-opaque Bodies and Fat Droplets in Mouse Liver after Fasting or Glucose Injection
Fasting produces an increased mobilization of lipid from adipose tissue to the liver and a decreased hepatic lipogenesis, but the administration of glucose stimulates lipid synthesis by the liver. After fasting of C3H mice numerous electron-opaque bodies and large lipid droplets were present in the liver. In the liver of untreated controls only a few small electron-opaque bodies and an occasion...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1970
ISSN: 0014-5793
DOI: 10.1016/0014-5793(70)80081-3